The importance of hydroxypyridine derivatives in different life activities has initiated us to propose the subject of the thesis. The thesis presents experimental spectral studies associated with theoretical quantum mechanical calculations of some important chemical behaviors of 3-Hydroxypyridine(3HP) and 4-Hydroxypyridine(4HP). Their preference geometry and structural data, their behavior as donors towards iodine (I2) and 2,3-dichloro-5,6-dicyano-pbenzo-quinone (DDQ) molecules, and their electronic transitions in the absorption spectra were investigated.